凯时66:少量NaCl缓解KCl胁迫对紫花苜蓿幼苗根系和叶片光合活性的影响

引用本文: 苗宇，张浩阳，张丽佳，刘美君，王爽. 少量NaCl缓解KCl胁迫对紫花苜蓿幼苗根系和叶片光合活性的影响. 草业科学, 2022, 39(5): 1-10 doi: shu

Citation: MIAO Y, ZHANG H Y, ZHANG L J, LIU M J, WANG S. Effects of small amounts of NaCl on alleviating damage caused to the photosynthetic activity of alfalfa seedling roots and leaves by KCl stress. Pratacultural Science, 2022, 39(5): 1-10 doi: shu

少量NaCl缓解KCl胁迫对紫花苜蓿幼苗根系和叶片光合活性的影响

新疆农业大学草业学院, 新疆乌鲁木齐 830052

作者简介: 苗宇(1996-)，男(蒙古族)，内蒙古太仆寺旗人，在读硕士生，研究方向为植物逆境生理。E-mail: 2542299781@qq.com

通讯作者: 刘美君(1988-)，女，山西五台人，副教授，博士，研究方向为牧草生理。E-mail: lebaby7@163.com

基金项目: 国家自然科学基金项目(3186130443)；财政部和农业农村部：国家现代农业产业技术体系自主

摘要: 新疆盐渍化土壤中富含多种类型盐离子，严重影响苜蓿生长，制约苜蓿产业在新疆的发展。本研究分析了不同比例混合的钠盐和钾盐对紫花苜蓿(Medicago sativa)幼苗根系生长以及光合活性的影响。结果表明：随着单盐NaCl、KCl浓度的升高，紫花苜蓿根系和幼叶电导率、丙二醛(MDA)和脯氨酸逐渐增加，根系活力、叶绿素含量以及叶片最大光化学效率(F_v/F_m)逐渐下降，且在相同浓度下KCl处理下降程度更大。这表明，盐胁迫破坏了苜蓿幼苗细胞膜完整性，导致膜脂过氧化严重，细胞自身的渗透调节作用不足以缓解盐胁迫对幼苗的影响，从而导致苜蓿幼苗根系活力下降，叶片光合效率受到影响，并且苜蓿幼苗在KCl胁迫下伤害更大。然而在高浓度KCl中加入少量NaCl，上述指标的变化较单独KCl处理有所下降，这表明少量Na⁺减轻了K⁺胁迫对细胞膜的损伤，减少了细胞的氧化伤害，增加了细胞的渗透调节作用，从而缓解了对根系活力的影响；此外，少量NaCl缓解了KCl胁迫下幼叶的氧化伤害，减少了叶绿素的降解，维持了叶片的光合活性。

关键词:

English

Effects of small amounts of NaCl on alleviating damage caused to the photosynthetic activity of alfalfa seedling roots and leaves by KCl stress

Xin Jiang Agricultural Universlty, Urumqi 830052, Xingjiang, China

Corresponding author: LIU Meijun　E-mail: lebaby7@163.com

Received Date: 2021-06-15
Accepted Date: 2021-12-29
Available Online: 2022-04-20

Abstract: Salinized soils in Xinjiang are rich in various types of salt ions, which significantly affect the growth of alfalfa (Medicago sativa) and restrict the development of the alfalfa industry in Xinjiang. In this study, the effects of different ratios of mixed sodium and potassium salts on the root growth and photosynthetic activity of alfalfa seedlings were investigated. The results showed that the electrical conductivity and malondialdehyde and proline levles of alfalfa roots and young leaves gradually increased while root vigor, chlorophyll content, and the maximum photochemical efficiency (F_v/F_m) of the leaves gradually decreased with increasing concentrations of NaCl and KCl alone, and the decrease was greater in the treatment with KCl alone at the same concentration. This suggests that salt stress disrupted the cell membrane integrity of alfalfa seedlings, leading to severe membrane lipid peroxidation, and that the osmoregulation of the cells itself reduced root vigor and photosynthetic efficiency, causing the seedlings to be more damaged by KCl stress. However, after a small amount of NaCl was added to the high concentration KCl treatment, the changes in the above indicators decreased compared with those observed in the group treated with KCl alone, which indicated that a small amount of Na⁺ ions alleviated the damage to the cell membrane caused by K⁺ ion stress, reduced the oxidative damage to the cell, and increased osmoregulation, thus alleviating the effect on root vigor. In addition, a small amount of NaCl alleviated the oxidative damage to young leaves under KCl stress and reduced the degradation of chlorophyll; thus, maintaining the photosynthetic activity of the leaves.

Key words:

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图 1 不同盐胁迫后紫花苜蓿根系电导率

Figure 1. Electrical conductivity of alfalfa root system after different salt stress treatments

图A同一处理不同小写字母表示NaCl和KCl间差异显著(P < 0.05)；图B不同小写字母表示不同处理间差异显著(P < 0.05)；下图同。

A. Lowercase letters within the same treatment indicate significant differences between NaCl and KCl at the 0.05 level; B. 200Na, 200 mmol·L⁻¹ NaCl; 20Na, 20 mmol·L⁻¹ NaCl; 200K, 200 mmol·L⁻¹ KCl; 20K, 20 mmol·L⁻¹ KCl; Different lowercase letters indicate significant differences between different treatments at the 0.05 level; this is applicable for the following figures as well.

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图 2 不同盐胁迫后紫花苜蓿根系丙二醛含量

Figure 2. Malondialdehyde content of alfalfa roots after different salt stress treatments

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图 3 不同盐胁迫后紫花苜蓿根系脯氨酸含量

Figure 3. Proline content of alfalfa root system after different salt stress treatments

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图 4 不同盐胁迫后紫花苜蓿根系活力

Figure 4. Root vigor of alfalfa after different salt stress treatments

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图 5 不同胁迫后紫花苜蓿各叶绿素含量的变化

Figure 5. Changes in the chlorophyll content of alfalfa after different treatments

同一浓度不同小写字母表示NaCl和KCl间差异显著(P < 0.05)。

Lowercase letters within the same concentration indicate significant differences between NaCl and KCl at the 0.05 level.

下载: 全尺寸图片幻灯片

图 6 混盐胁迫后紫花苜蓿叶绿素含量的变化

Figure 6. Changes in the chlorophyll content of alfalfa after mixed salt stress

同一指标不同小写字母表示各处理间差异显著(P＜0.05)。

Different lowercase letters within the same index indicate significant differences between different treatments at the 0.05 level.

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图 7 不同盐胁迫后紫花苜蓿类胡萝卜素含量的变化

Figure 7. Carotenoid content of alfalfa after different salt stress treatments

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图 8 不同盐胁迫后紫花苜蓿F_v/F_m的变化

Figure 8. Changes in F_v/F_m of alfalfa after different salt stress treatments

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图 9 不同盐胁迫后紫花苜蓿叶片脯氨酸含量

Figure 9. Proline content of alfalfa leaves after different salt stress treatments

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